求助Rosetta显示蛋白序列位置为0报错

bht

您好。我是rosetta小白，想要用其进行氨基酸突变，运行代码如下
/public/software/.local/easybuild/software/ROSETTA/rosetta_bin_linux_2021.16.61629_bundle/main/source/bin/pmut_scan_parallel.static.linuxgccrelease -database /public/software/.local/easybuild/software/ROSETTA/rosetta_bin_linux_2021.16.61629_bundle/main/database -s 160ns0.pdb -mutants_list mut -output_mutant_structures -ex1 -ex2 -ex3 -extrachi_cutoff 1 -use_input_sc -ignore_unrecognized_res -no_his_his_pairE -multi_cool_annealer 10 -mute basic core -ignore_zero_occupancy false
报错如下
请问如何解决呢？
烦请大家解答一下

linlinlin

请问你后来有解决这个问题吗？